Branched oligonucleotides
WebSep 8, 2003 · Branched oligonucleotides (b-oligonucleotides) based on a novel branching monomer were used for site-specific sequence alteration in vivo. With a stable integrated mutated enhanced green ... WebNov 26, 2013 · In a recent article (Gudlur et al. PLOS ONE, 2012, 7 (9) e45374), we described the special properties of a mixed branched peptide assembly in which equimolar bis (FLIVI)-K-KKKK and bis (FLIVIGSII)-K-KKKK self-associate to form bilayer delimited capsules capable of trapping solutes. These polycationic vesicle-like capsules are readily …
Branched oligonucleotides
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WebJul 8, 2024 · A DMT-protected alcohol (3g) was prepared for making branched oligonucleotides. Sulfonyl azides were also considered for incorporation of fluorophores into oligonucleotides and for this purpose we synthesised two sulfonyl azides fluorophores, pyrene ( 3h ) and dansyl ( 3i ). WebApr 11, 1995 · All the oligonucleotides prepared contained two T11 sequences, and the branched and looped oligomers an additional alternating CT sequence. The melting experiments revealed that the branched oligonucleotides form relatively weak hybrid (double/triple helix) complexes with the single-stranded oligodeoxyribonucleotide, …
Webintermediates contain almost exclusively adenosine at the branch point; therefore, the protocol given below describes the synthesis of the adenosine branching phosphoramidite synthon (BIS-A; S.3; Fig. 4.14.1) used for the synthesis of symmetrical, branched DNA and RNA oligonucleotides (Damha and Ogilvie, 1988). The same protocol may be WebOct 8, 2024 · Nucleoside phosphoramidite derivatives containing two protected primary hydroxyl functions have been incorporated into synthetic oligonucleotides as 'branching monomers'. With selective deprotection, multiple identical copies of an additional oligonucleotide can be incorporated to form fork- or comb-like structures for use as signal ...
WebFeb 1, 2006 · Oligonucleotides used as primers for the polymerase chain reaction (PCR) assay were found to perform identically to control primers, demonstrating full biological compatibility. WebJun 9, 2006 · During the synthesis of oligonucleotides by the standard phosphoramidite method using 2′-deoxycytidine- derivatized solid support, a side reaction was observed that gave rise to the formation of high molecular weight N-branched oligomers having two identical chains linked to the 3′-terminal 2′-deoxycytidine.Postsynthesis treatment with …
WebFinally, branched oligonucleotides have been used to produce V-shape oligonucleotides with high affinity to singlestranded DNA or RNA by triplex formation [9,14,15,17,21,23, 24] and to form ...
WebFeb 1, 2004 · Attempted replication of immobilized branched 5 ¢ -radiolabeled oligonucleotides across the 2 ¢ ±5 and the 3 ¢ ±5 ¢ linkages at the branch point. Comparison between f 29 DNA polymerase and ... pargono o paionoWebThe unique architecture of branched oligonucleotides mimicking lariat RNA introns [Wallace and Edmons, Proc. Natl. Acad. Sci. USA 80, 950-954 (1983)] was exploited to study compounds that associate as two parallel duplexes with intercalating C/C+ base pairs (i-motif DNA) [Gehring et al. Nature 363, … オペラハウス 岡山Web[47,48] Beside this modification, branched oligonucleotides [49] and dumbbell-shaped circular RNAs [50][51][52] have been developed with excellent resistance to 3'-exonucleases. Recently, a ... par gol siciliaWebFinally, branched oligonucleotides have been used to produce V-shape oligonucleotides with high affinity to singlestranded DNA or RNA by triplex formation [9,14,15,17,21,23, 24] and to form ... par gol regione piemonteWebAbstract. PEGylation, the attachment of poly (ethylene glycol) (PEG), has been adopted to improve the pharmacokinetic properties of oligonucleotide therapeutics for nearly 30 years. Prior efforts mainly focused on the investigation of linear or slightly branched PEG having different molecular weights, terminal functional groups, and possible ... par golferWebSolid-phase synthesis of branched oligonucleotides containing a biologically relevant dCyd341 interstrand crosslink DNA lesion 1 Author links open overlay panel Marisa L. Taverna Porro 2 a , Christine Saint-Pierre a , Didier Gasparutto a , Jean-Luc Ravanat a オペラハウス 夜WebNovel antisense oligonucleotides are disclosed. The present oligonucleotides have increased resistance to nuclease attack and manifest increased efficacy in combating pathogenic infections. The present oligonucleotides comprise two or more identical or different sequences, each complementary to a nucleic acid sequence of a pathogen that … par gol sardegna